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How do I look up information about an enzyme?

Program name: redata     

(molbiol username / password required)

Notes: This program shows information about restriction enzymes including references, recognition and cleavage sites, isoschizomers, commercial availability and methylation sensitivity (Note, does not include Type IV enzymes).

Output from redata regarding EcoRV

EcoRV 

Recognition site is GATATC leaving blunt ends
  Cut positions 5':3 3':3
Organism: Escherichia coli J62 pLG74
Methylated: 2(6)
Source: L.I. Glatman

Isoschizomers:
   Bsc217I     BshLI       BsoAI       Bsp16I      BstRI       CeqI        
   Eco17I      Eco32I      Eco178I     HjaI        HpyCI       NanI        
   NflAI       NsiCI       Pac1110II   Pfl16I      Rma495II    Rma496II    
   Rma497II    SmiMII      Tsp273I     Uba1400I    
   
Suppliers:
GE Healthcare (8/05)
Invitrogen Corporation (8/05)
Minotech Biotechnology (9/05)
Qbiogene (9/05)
American Allied Biochemical, Inc. (9/05)
SibEnzyme Ltd. (8/05)
Nippon Gene Co., Ltd. (8/05)
Takara Bio Inc. (9/05)
Roche Applied Science (8/05)
New England Biolabs (9/05)
Toyobo Biochemicals (9/05)
Molecular Biology Resources (8/05)
Promega Corporation (9/05)
Sigma Chemical Corporation (9/05)
Bangalore Genei (9/05)
EURx Ltd. (9/05)
CinnaGen Inc. (9/05)

References:
Baldwin, G.S., Halford, S.E., (1994) Biochem. Soc. Trans., vol. 22, pp. 300S.
Erskine, S.G., Halford, S.E., (1994) Biochem. Soc. Trans., vol. 22, pp. 299s.
Forrow, S., Lee, M., Souhami, R.L., Hartley, J.A., (1994) ACS Abstracts, 
   vol. 208, pp. 97.
Kholmina, G.V., Rebentish, B.A., Skoblov, Y.S., Mironov, A.A., Yankovskii, 
   N.K., Kozlov, Y.I., Glatmann, L.I., Moroz, A.F., Debabov, V.G., (1980) 
   Dokl. Akad. Nauk., vol. 253, pp. 495-497.
Nwosu, V.U., Connolly, B.A., Halford, S.E., Garnett, J., (1988) Nucleic Acids 
   Res., vol. 16, pp. 3705-3720.
Schildkraut, I., Banner, C.D.B., Rhodes, C.S., Parekh, S., (1984) Gene, vol.
   27, pp. 327-329.
Vipond, I.B., Moon, B.J., Halford, S.E., (1994) Biochem. Soc. Trans., vol.
   22, pp. 301S.
Winkler, F.K., (1994) J. Mol. Recognit., vol. 6, pp. 9.

Further reading: redata background


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This file last modified Friday November 18, 2011